SrasV1, Main, Exploration, bibRecord, 001877

Rapid Generation of Human-Like Neutralizing Monoclonal Antibodies in Urgent Preparedness for Influenza Pandemics and Virulent Infectious Diseases

Identifieur interne : 001877 ( Main/Exploration ); précédent : 001876; suivant : 001878

Rapid Generation of Human-Like Neutralizing Monoclonal Antibodies in Urgent Preparedness for Influenza Pandemics and Virulent Infectious Diseases

Source :

PLoS ONE [ 1932-6203 ] ; 2013.

RBID : PMC:3688872

Descripteurs français

KwdFr :
- Animaux, Anticorps monoclonaux (biosynthèse), Anticorps monoclonaux (immunologie), Anticorps neutralisants (biosynthèse), Anticorps neutralisants (immunologie), Flambées de maladies, Grippe humaine (), Grippe humaine (immunologie), Grippe humaine (épidémiologie), Humains, Macaca mulatta, Maladies transmissibles émergentes (), Maladies transmissibles émergentes (immunologie), Maladies transmissibles émergentes (épidémiologie), Souris, Sous-type H5N1 du virus de la grippe A (immunologie).
MESH :
- biosynthèse : Anticorps monoclonaux, Anticorps neutralisants.
- immunologie : Anticorps monoclonaux, Anticorps neutralisants, Grippe humaine, Maladies transmissibles émergentes, Sous-type H5N1 du virus de la grippe A.
- épidémiologie : Grippe humaine, Maladies transmissibles émergentes.
- Animaux, Flambées de maladies, Grippe humaine, Humains, Macaca mulatta, Maladies transmissibles émergentes, Souris.

English descriptors

KwdEn :
- Animals, Antibodies, Monoclonal (biosynthesis), Antibodies, Monoclonal (immunology), Antibodies, Neutralizing (biosynthesis), Antibodies, Neutralizing (immunology), Communicable Diseases, Emerging (epidemiology), Communicable Diseases, Emerging (immunology), Communicable Diseases, Emerging (therapy), Disease Outbreaks, Humans, Influenza A Virus, H5N1 Subtype (immunology), Influenza, Human (epidemiology), Influenza, Human (immunology), Influenza, Human (therapy), Macaca mulatta, Mice.
MESH :
- chemical , biosynthesis : Antibodies, Monoclonal, Antibodies, Neutralizing.
- chemical , immunology : Antibodies, Monoclonal, Antibodies, Neutralizing.
- epidemiology : Communicable Diseases, Emerging, Influenza, Human.
- immunology : Communicable Diseases, Emerging, Influenza A Virus, H5N1 Subtype, Influenza, Human.
- therapy : Communicable Diseases, Emerging, Influenza, Human.
- Animals, Disease Outbreaks, Humans, Macaca mulatta, Mice.

Abstract

Background

The outbreaks of emerging infectious diseases caused by pathogens such as SARS coronavirus, H5N1, H1N1, and recently H7N9 influenza viruses, have been associated with significant mortality and morbidity in humans. Neutralizing antibodies from individuals who have recovered from an infection confer therapeutic protection to others infected with the same pathogen. However, survivors may not always be available for providing plasma or for the cloning of monoclonal antibodies (mAbs).

Methodology/Principal Findings

The genome and the immunoglobulin genes in rhesus macaques and humans are highly homologous; therefore, we investigated whether neutralizing mAbs that are highly homologous to those of humans (human-like) could be generated. Using the H5N1 influenza virus as a model, we first immunized rhesus macaques with recombinant adenoviruses carrying a synthetic gene encoding hemagglutinin (HA). Following screening an antibody phage display library derived from the B cells of immunized monkeys, we cloned selected macaque immunoglobulin heavy chain and light chain variable regions into the human IgG constant region, which generated human-macaque chimeric mAbs exhibiting over 97% homology to human antibodies. Selected mAbs demonstrated potent neutralizing activities against three clades (0, 1, 2) of the H5N1 influenza viruses. The in vivo protection experiments demonstrated that the mAbs effectively protected the mice even when administered up to 3 days after infection with H5N1 influenza virus. In particular, mAb 4E6 demonstrated sub-picomolar binding affinity to HA and superior in vivo protection efficacy without the loss of body weight and obvious lung damage. The analysis of the 4E6 escape mutants demonstrated that the 4E6 antibody bound to a conserved epitope region containing two amino acids on the globular head of HA.

Conclusions/Significance

Our study demonstrated the generation of neutralizing mAbs for potential application in humans in urgent preparedness against outbreaks of new influenza infections or other virulent infectious diseases.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3688872

DOI: 10.1371/journal.pone.0066276
PubMed: 23824680
PubMed Central: 3688872

Affiliations:

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Rapid Generation of Human-Like Neutralizing Monoclonal Antibodies in Urgent Preparedness for Influenza Pandemics and Virulent Infectious Diseases</title>
<author><name sortKey="Meng, Weixu" sort="Meng, Weixu" uniqKey="Meng W" first="Weixu" last="Meng">Weixu Meng</name>
<affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
<affiliation wicri:level="3"><nlm:aff id="aff2"> The<addr-line>First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>First Affiliated Hospital of Guangzhou Medical University, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Pan, Weiqi" sort="Pan, Weiqi" uniqKey="Pan W" first="Weiqi" last="Pan">Weiqi Pan</name>
<affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
<affiliation wicri:level="3"><nlm:aff id="aff2"> The<addr-line>First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>First Affiliated Hospital of Guangzhou Medical University, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Zhang, Anna J X" sort="Zhang, Anna J X" uniqKey="Zhang A" first="Anna J. X." last="Zhang">Anna J. X. Zhang</name>
<affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Department of Microbiology, State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Microbiology, State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Li, Zhengfeng" sort="Li, Zhengfeng" uniqKey="Li Z" first="Zhengfeng" last="Li">Zhengfeng Li</name>
<affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
<affiliation wicri:level="3"><nlm:aff id="aff2"> The<addr-line>First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>First Affiliated Hospital of Guangzhou Medical University, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Wei, Guowei" sort="Wei, Guowei" uniqKey="Wei G" first="Guowei" last="Wei">Guowei Wei</name>
<affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Feng, Liqiang" sort="Feng, Liqiang" uniqKey="Feng L" first="Liqiang" last="Feng">Liqiang Feng</name>
<affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
<affiliation wicri:level="3"><nlm:aff id="aff2"> The<addr-line>First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>First Affiliated Hospital of Guangzhou Medical University, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Dong, Zhenyuan" sort="Dong, Zhenyuan" uniqKey="Dong Z" first="Zhenyuan" last="Dong">Zhenyuan Dong</name>
<affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
<affiliation wicri:level="3"><nlm:aff id="aff2"> The<addr-line>First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>First Affiliated Hospital of Guangzhou Medical University, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Li, Chufang" sort="Li, Chufang" uniqKey="Li C" first="Chufang" last="Li">Chufang Li</name>
<affiliation wicri:level="3"><nlm:aff id="aff2"> The<addr-line>First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>First Affiliated Hospital of Guangzhou Medical University, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hu, Xiangjing" sort="Hu, Xiangjing" uniqKey="Hu X" first="Xiangjing" last="Hu">Xiangjing Hu</name>
<affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Sun, Caijun" sort="Sun, Caijun" uniqKey="Sun C" first="Caijun" last="Sun">Caijun Sun</name>
<affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
<affiliation wicri:level="3"><nlm:aff id="aff2"> The<addr-line>First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>First Affiliated Hospital of Guangzhou Medical University, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Luo, Qinfang" sort="Luo, Qinfang" uniqKey="Luo Q" first="Qinfang" last="Luo">Qinfang Luo</name>
<affiliation wicri:level="3"><nlm:aff id="aff2"> The<addr-line>First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>First Affiliated Hospital of Guangzhou Medical University, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name>
<affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Department of Microbiology, State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Microbiology, State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhong, Nanshan" sort="Zhong, Nanshan" uniqKey="Zhong N" first="Nanshan" last="Zhong">Nanshan Zhong</name>
<affiliation wicri:level="3"><nlm:aff id="aff2"> The<addr-line>First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>First Affiliated Hospital of Guangzhou Medical University, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chen, Ling" sort="Chen, Ling" uniqKey="Chen L" first="Ling" last="Chen">Ling Chen</name>
<affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
<affiliation wicri:level="3"><nlm:aff id="aff2"> The<addr-line>First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>First Affiliated Hospital of Guangzhou Medical University, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">23824680</idno>
<idno type="pmc">3688872</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3688872</idno>
<idno type="RBID">PMC:3688872</idno>
<idno type="doi">10.1371/journal.pone.0066276</idno>
<date when="2013">2013</date>
<idno type="wicri:Area/Pmc/Corpus">001385</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001385</idno>
<idno type="wicri:Area/Pmc/Curation">001385</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001385</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000957</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000957</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="RBID">pubmed:23824680</idno>
<idno type="wicri:Area/PubMed/Corpus">001173</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001173</idno>
<idno type="wicri:Area/PubMed/Curation">001173</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001173</idno>
<idno type="wicri:Area/PubMed/Checkpoint">001118</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001118</idno>
<idno type="wicri:Area/Ncbi/Merge">002701</idno>
<idno type="wicri:Area/Ncbi/Curation">002701</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">002701</idno>
<idno type="wicri:Area/Main/Merge">001883</idno>
<idno type="wicri:Area/Main/Curation">001877</idno>
<idno type="wicri:Area/Main/Exploration">001877</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Rapid Generation of Human-Like Neutralizing Monoclonal Antibodies in Urgent Preparedness for Influenza Pandemics and Virulent Infectious Diseases</title>
<author><name sortKey="Meng, Weixu" sort="Meng, Weixu" uniqKey="Meng W" first="Weixu" last="Meng">Weixu Meng</name>
<affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
<affiliation wicri:level="3"><nlm:aff id="aff2"> The<addr-line>First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>First Affiliated Hospital of Guangzhou Medical University, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Pan, Weiqi" sort="Pan, Weiqi" uniqKey="Pan W" first="Weiqi" last="Pan">Weiqi Pan</name>
<affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
<affiliation wicri:level="3"><nlm:aff id="aff2"> The<addr-line>First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>First Affiliated Hospital of Guangzhou Medical University, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Zhang, Anna J X" sort="Zhang, Anna J X" uniqKey="Zhang A" first="Anna J. X." last="Zhang">Anna J. X. Zhang</name>
<affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Department of Microbiology, State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Microbiology, State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Li, Zhengfeng" sort="Li, Zhengfeng" uniqKey="Li Z" first="Zhengfeng" last="Li">Zhengfeng Li</name>
<affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
<affiliation wicri:level="3"><nlm:aff id="aff2"> The<addr-line>First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>First Affiliated Hospital of Guangzhou Medical University, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Wei, Guowei" sort="Wei, Guowei" uniqKey="Wei G" first="Guowei" last="Wei">Guowei Wei</name>
<affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Feng, Liqiang" sort="Feng, Liqiang" uniqKey="Feng L" first="Liqiang" last="Feng">Liqiang Feng</name>
<affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
<affiliation wicri:level="3"><nlm:aff id="aff2"> The<addr-line>First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>First Affiliated Hospital of Guangzhou Medical University, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Dong, Zhenyuan" sort="Dong, Zhenyuan" uniqKey="Dong Z" first="Zhenyuan" last="Dong">Zhenyuan Dong</name>
<affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
<affiliation wicri:level="3"><nlm:aff id="aff2"> The<addr-line>First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>First Affiliated Hospital of Guangzhou Medical University, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Li, Chufang" sort="Li, Chufang" uniqKey="Li C" first="Chufang" last="Li">Chufang Li</name>
<affiliation wicri:level="3"><nlm:aff id="aff2"> The<addr-line>First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>First Affiliated Hospital of Guangzhou Medical University, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hu, Xiangjing" sort="Hu, Xiangjing" uniqKey="Hu X" first="Xiangjing" last="Hu">Xiangjing Hu</name>
<affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Sun, Caijun" sort="Sun, Caijun" uniqKey="Sun C" first="Caijun" last="Sun">Caijun Sun</name>
<affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
<affiliation wicri:level="3"><nlm:aff id="aff2"> The<addr-line>First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>First Affiliated Hospital of Guangzhou Medical University, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Luo, Qinfang" sort="Luo, Qinfang" uniqKey="Luo Q" first="Qinfang" last="Luo">Qinfang Luo</name>
<affiliation wicri:level="3"><nlm:aff id="aff2"> The<addr-line>First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>First Affiliated Hospital of Guangzhou Medical University, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name>
<affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Department of Microbiology, State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Microbiology, State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhong, Nanshan" sort="Zhong, Nanshan" uniqKey="Zhong N" first="Nanshan" last="Zhong">Nanshan Zhong</name>
<affiliation wicri:level="3"><nlm:aff id="aff2"> The<addr-line>First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>First Affiliated Hospital of Guangzhou Medical University, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chen, Ling" sort="Chen, Ling" uniqKey="Chen L" first="Ling" last="Chen">Ling Chen</name>
<affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
<affiliation wicri:level="3"><nlm:aff id="aff2"> The<addr-line>First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>First Affiliated Hospital of Guangzhou Medical University, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">PLoS ONE</title>
<idno type="eISSN">1932-6203</idno>
<imprint><date when="2013">2013</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Antibodies, Monoclonal (biosynthesis)</term>
<term>Antibodies, Monoclonal (immunology)</term>
<term>Antibodies, Neutralizing (biosynthesis)</term>
<term>Antibodies, Neutralizing (immunology)</term>
<term>Communicable Diseases, Emerging (epidemiology)</term>
<term>Communicable Diseases, Emerging (immunology)</term>
<term>Communicable Diseases, Emerging (therapy)</term>
<term>Disease Outbreaks</term>
<term>Humans</term>
<term>Influenza A Virus, H5N1 Subtype (immunology)</term>
<term>Influenza, Human (epidemiology)</term>
<term>Influenza, Human (immunology)</term>
<term>Influenza, Human (therapy)</term>
<term>Macaca mulatta</term>
<term>Mice</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Anticorps monoclonaux (biosynthèse)</term>
<term>Anticorps monoclonaux (immunologie)</term>
<term>Anticorps neutralisants (biosynthèse)</term>
<term>Anticorps neutralisants (immunologie)</term>
<term>Flambées de maladies</term>
<term>Grippe humaine ()</term>
<term>Grippe humaine (immunologie)</term>
<term>Grippe humaine (épidémiologie)</term>
<term>Humains</term>
<term>Macaca mulatta</term>
<term>Maladies transmissibles émergentes ()</term>
<term>Maladies transmissibles émergentes (immunologie)</term>
<term>Maladies transmissibles émergentes (épidémiologie)</term>
<term>Souris</term>
<term>Sous-type H5N1 du virus de la grippe A (immunologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Antibodies, Monoclonal</term>
<term>Antibodies, Neutralizing</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term>
<term>Antibodies, Neutralizing</term>
</keywords>
<keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Anticorps monoclonaux</term>
<term>Anticorps neutralisants</term>
</keywords>
<keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Communicable Diseases, Emerging</term>
<term>Influenza, Human</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps monoclonaux</term>
<term>Anticorps neutralisants</term>
<term>Grippe humaine</term>
<term>Maladies transmissibles émergentes</term>
<term>Sous-type H5N1 du virus de la grippe A</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Communicable Diseases, Emerging</term>
<term>Influenza A Virus, H5N1 Subtype</term>
<term>Influenza, Human</term>
</keywords>
<keywords scheme="MESH" qualifier="therapy" xml:lang="en"><term>Communicable Diseases, Emerging</term>
<term>Influenza, Human</term>
</keywords>
<keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Grippe humaine</term>
<term>Maladies transmissibles émergentes</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Disease Outbreaks</term>
<term>Humans</term>
<term>Macaca mulatta</term>
<term>Mice</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Flambées de maladies</term>
<term>Grippe humaine</term>
<term>Humains</term>
<term>Macaca mulatta</term>
<term>Maladies transmissibles émergentes</term>
<term>Souris</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><sec sec-type="headed"><title>Background</title>
<p>The outbreaks of emerging infectious diseases caused by pathogens such as SARS coronavirus, H5N1, H1N1, and recently H7N9 influenza viruses, have been associated with significant mortality and morbidity in humans. Neutralizing antibodies from individuals who have recovered from an infection confer therapeutic protection to others infected with the same pathogen. However, survivors may not always be available for providing plasma or for the cloning of monoclonal antibodies (mAbs).</p>
</sec>
<sec sec-type="headed"><title>Methodology/Principal Findings</title>
<p>The genome and the immunoglobulin genes in rhesus macaques and humans are highly homologous; therefore, we investigated whether neutralizing mAbs that are highly homologous to those of humans (human-like) could be generated. Using the H5N1 influenza virus as a model, we first immunized rhesus macaques with recombinant adenoviruses carrying a synthetic gene encoding hemagglutinin (HA). Following screening an antibody phage display library derived from the B cells of immunized monkeys, we cloned selected macaque immunoglobulin heavy chain and light chain variable regions into the human IgG constant region, which generated human-macaque chimeric mAbs exhibiting over 97% homology to human antibodies. Selected mAbs demonstrated potent neutralizing activities against three clades (0, 1, 2) of the H5N1 influenza viruses. The <italic>in vivo</italic>
 protection experiments demonstrated that the mAbs effectively protected the mice even when administered up to 3 days after infection with H5N1 influenza virus. In particular, mAb 4E6 demonstrated sub-picomolar binding affinity to HA and superior <italic>in vivo</italic>
 protection efficacy without the loss of body weight and obvious lung damage. The analysis of the 4E6 escape mutants demonstrated that the 4E6 antibody bound to a conserved epitope region containing two amino acids on the globular head of HA.</p>
</sec>
<sec sec-type="headed"><title>Conclusions/Significance</title>
<p>Our study demonstrated the generation of neutralizing mAbs for potential application in humans in urgent preparedness against outbreaks of new influenza infections or other virulent infectious diseases.</p>
</sec>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Zhou, B" uniqKey="Zhou B">B Zhou</name>
</author>
<author><name sortKey="Zhong, N" uniqKey="Zhong N">N Zhong</name>
</author>
<author><name sortKey="Guan, Y" uniqKey="Guan Y">Y Guan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hung, If" uniqKey="Hung I">IF Hung</name>
</author>
<author><name sortKey="To, Kk" uniqKey="To K">KK To</name>
</author>
<author><name sortKey="Lee, Ck" uniqKey="Lee C">CK Lee</name>
</author>
<author><name sortKey="Lee, Kl" uniqKey="Lee K">KL Lee</name>
</author>
<author><name sortKey="Chan, K" uniqKey="Chan K">K Chan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kashyap, Ak" uniqKey="Kashyap A">AK Kashyap</name>
</author>
<author><name sortKey="Steel, J" uniqKey="Steel J">J Steel</name>
</author>
<author><name sortKey="Oner, Af" uniqKey="Oner A">AF Oner</name>
</author>
<author><name sortKey="Dillon, Ma" uniqKey="Dillon M">MA Dillon</name>
</author>
<author><name sortKey="Swale, Re" uniqKey="Swale R">RE Swale</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wrammert, J" uniqKey="Wrammert J">J Wrammert</name>
</author>
<author><name sortKey="Smith, K" uniqKey="Smith K">K Smith</name>
</author>
<author><name sortKey="Miller, J" uniqKey="Miller J">J Miller</name>
</author>
<author><name sortKey="Langley, Wa" uniqKey="Langley W">WA Langley</name>
</author>
<author><name sortKey="Kokko, K" uniqKey="Kokko K">K Kokko</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yoshida, R" uniqKey="Yoshida R">R Yoshida</name>
</author>
<author><name sortKey="Igarashi, M" uniqKey="Igarashi M">M Igarashi</name>
</author>
<author><name sortKey="Ozaki, H" uniqKey="Ozaki H">H Ozaki</name>
</author>
<author><name sortKey="Kishida, N" uniqKey="Kishida N">N Kishida</name>
</author>
<author><name sortKey="Tomabechi, D" uniqKey="Tomabechi D">D Tomabechi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sun, L" uniqKey="Sun L">L Sun</name>
</author>
<author><name sortKey="Lu, X" uniqKey="Lu X">X Lu</name>
</author>
<author><name sortKey="Li, C" uniqKey="Li C">C Li</name>
</author>
<author><name sortKey="Wang, M" uniqKey="Wang M">M Wang</name>
</author>
<author><name sortKey="Liu, Q" uniqKey="Liu Q">Q Liu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Throsby, M" uniqKey="Throsby M">M Throsby</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yu, X" uniqKey="Yu X">X Yu</name>
</author>
<author><name sortKey="Tsibane, T" uniqKey="Tsibane T">T Tsibane</name>
</author>
<author><name sortKey="Mcgraw, Pa" uniqKey="Mcgraw P">PA McGraw</name>
</author>
<author><name sortKey="House, Fs" uniqKey="House F">FS House</name>
</author>
<author><name sortKey="Keefer, Cj" uniqKey="Keefer C">CJ Keefer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sui, J" uniqKey="Sui J">J Sui</name>
</author>
<author><name sortKey="Hwang, Wc" uniqKey="Hwang W">WC Hwang</name>
</author>
<author><name sortKey="Perez, S" uniqKey="Perez S">S Perez</name>
</author>
<author><name sortKey="Wei, G" uniqKey="Wei G">G Wei</name>
</author>
<author><name sortKey="Aird, D" uniqKey="Aird D">D Aird</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Corti, D" uniqKey="Corti D">D Corti</name>
</author>
<author><name sortKey="Suguitan Pinna, D Jr" uniqKey="Suguitan Pinna D">D Jr Suguitan Pinna</name>
</author>
<author><name sortKey="Pinna D" uniqKey="Pinna D">Pinna D</name>
</author>
<author><name sortKey="Fernandez Rodriguez, Bm" uniqKey="Fernandez Rodriguez B">BM Fernandez-Rodriguez</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Simmons, Cp" uniqKey="Simmons C">CP Simmons</name>
</author>
<author><name sortKey="Bernasconi, Nl" uniqKey="Bernasconi N">NL Bernasconi</name>
</author>
<author><name sortKey="Suguitan, Al" uniqKey="Suguitan A">AL Suguitan</name>
</author>
<author><name sortKey="Mills, K" uniqKey="Mills K">K Mills</name>
</author>
<author><name sortKey="Ward, Jm" uniqKey="Ward J">JM Ward</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ekiert, Dc" uniqKey="Ekiert D">DC Ekiert</name>
</author>
<author><name sortKey="Bhabha, G" uniqKey="Bhabha G">G Bhabha</name>
</author>
<author><name sortKey="Elsliger, Ma" uniqKey="Elsliger M">MA Elsliger</name>
</author>
<author><name sortKey="Friesen, Rh" uniqKey="Friesen R">RH Friesen</name>
</author>
<author><name sortKey="Jongeneelen, M" uniqKey="Jongeneelen M">M Jongeneelen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Corti, D" uniqKey="Corti D">D Corti</name>
</author>
<author><name sortKey="Voss, J" uniqKey="Voss J">J Voss</name>
</author>
<author><name sortKey="Gamblin, Sj" uniqKey="Gamblin S">SJ Gamblin</name>
</author>
<author><name sortKey="Codoni, G" uniqKey="Codoni G">G Codoni</name>
</author>
<author><name sortKey="Macagno, A" uniqKey="Macagno A">A Macagno</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sundling, C" uniqKey="Sundling C">C Sundling</name>
</author>
<author><name sortKey="Li, Y" uniqKey="Li Y">Y Li</name>
</author>
<author><name sortKey="Huynh, N" uniqKey="Huynh N">N Huynh</name>
</author>
<author><name sortKey="Poulsen, C" uniqKey="Poulsen C">C Poulsen</name>
</author>
<author><name sortKey="Wilson, R" uniqKey="Wilson R">R Wilson</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="De Jong, Md" uniqKey="De Jong M">MD de Jong</name>
</author>
<author><name sortKey="Tran, Tt" uniqKey="Tran T">TT Tran</name>
</author>
<author><name sortKey="Khanh, Th" uniqKey="Khanh T">TH Khanh</name>
</author>
<author><name sortKey="Hien, Vm" uniqKey="Hien V">VM Hien</name>
</author>
<author><name sortKey="Smith, Gjd" uniqKey="Smith G">GJD Smith</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zheng, Bj" uniqKey="Zheng B">BJ Zheng</name>
</author>
<author><name sortKey="Chan, Kw" uniqKey="Chan K">KW Chan</name>
</author>
<author><name sortKey="Lin, Yp" uniqKey="Lin Y">YP Lin</name>
</author>
<author><name sortKey="Zhao, Gy" uniqKey="Zhao G">GY Zhao</name>
</author>
<author><name sortKey="Chan, C" uniqKey="Chan C">C Chan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hu, X" uniqKey="Hu X">X Hu</name>
</author>
<author><name sortKey="Meng, W" uniqKey="Meng W">W Meng</name>
</author>
<author><name sortKey="Dong, Z" uniqKey="Dong Z">Z Dong</name>
</author>
<author><name sortKey="Pan, W" uniqKey="Pan W">W Pan</name>
</author>
<author><name sortKey="Sun, C" uniqKey="Sun C">C Sun</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wei, Cj" uniqKey="Wei C">CJ Wei</name>
</author>
<author><name sortKey="Boyington, Jc" uniqKey="Boyington J">JC Boyington</name>
</author>
<author><name sortKey="Mctamney, Pm" uniqKey="Mctamney P">PM McTamney</name>
</author>
<author><name sortKey="Kong, Wp" uniqKey="Kong W">WP Kong</name>
</author>
<author><name sortKey="Pearce, Mb" uniqKey="Pearce M">MB Pearce</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hoelscher, Ma" uniqKey="Hoelscher M">MA Hoelscher</name>
</author>
<author><name sortKey="Garg, S" uniqKey="Garg S">S Garg</name>
</author>
<author><name sortKey="Bangari, Ds" uniqKey="Bangari D">DS Bangari</name>
</author>
<author><name sortKey="Belser, Ja" uniqKey="Belser J">JA Belser</name>
</author>
<author><name sortKey="Lu, X" uniqKey="Lu X">X Lu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bible, Jm" uniqKey="Bible J">JM Bible</name>
</author>
<author><name sortKey="Howard, W" uniqKey="Howard W">W Howard</name>
</author>
<author><name sortKey="Robbins, H" uniqKey="Robbins H">H Robbins</name>
</author>
<author><name sortKey="Dunn Walters, Dk" uniqKey="Dunn Walters D">DK Dunn-Walters</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Helmuth, Ef" uniqKey="Helmuth E">EF Helmuth</name>
</author>
<author><name sortKey="Letvin, Nl" uniqKey="Letvin N">NL Letvin</name>
</author>
<author><name sortKey="Margolin, Dh" uniqKey="Margolin D">DH Margolin</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Andris, Js" uniqKey="Andris J">JS Andris</name>
</author>
<author><name sortKey="Miller, Ab" uniqKey="Miller A">AB Miller</name>
</author>
<author><name sortKey="Abraham, Sr" uniqKey="Abraham S">SR Abraham</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Link, Jm" uniqKey="Link J">JM Link</name>
</author>
<author><name sortKey="Hellinger, Ma" uniqKey="Hellinger M">MA Hellinger</name>
</author>
<author><name sortKey="Schroeder, Hw" uniqKey="Schroeder H">HW Schroeder</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Howard, Wa" uniqKey="Howard W">WA Howard</name>
</author>
<author><name sortKey="Bible, Jm" uniqKey="Bible J">JM Bible</name>
</author>
<author><name sortKey="Finlay Dijsselbloem, E" uniqKey="Finlay Dijsselbloem E">E Finlay-Dijsselbloem</name>
</author>
<author><name sortKey="Openshaw, S" uniqKey="Openshaw S">S Openshaw</name>
</author>
<author><name sortKey="Dunn Walters, Dk" uniqKey="Dunn Walters D">DK Dunn-Walters</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Howard, Wa" uniqKey="Howard W">WA Howard</name>
</author>
<author><name sortKey="Bibke, Jm" uniqKey="Bibke J">JM Bibke</name>
</author>
<author><name sortKey="Finlay Dijsselbloem, E" uniqKey="Finlay Dijsselbloem E">E Finlay-Dijsselbloem</name>
</author>
<author><name sortKey="Openshaw, S" uniqKey="Openshaw S">S Openshaw</name>
</author>
<author><name sortKey="Dunn Walters, Dk" uniqKey="Dunn Walters D">DK Dunn-Walters</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Spencer, J" uniqKey="Spencer J">J Spencer</name>
</author>
<author><name sortKey="Dunn, M" uniqKey="Dunn M">M Dunn</name>
</author>
<author><name sortKey="Dunn Walters, Dk" uniqKey="Dunn Walters D">DK Dunn-Walters</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Margolin, Dh" uniqKey="Margolin D">DH Margolin</name>
</author>
<author><name sortKey="Saunders, Eh" uniqKey="Saunders E">EH Saunders</name>
</author>
<author><name sortKey="Bronfin, B" uniqKey="Bronfin B">B Bronfin</name>
</author>
<author><name sortKey="De Rosa, N" uniqKey="De Rosa N">N de Rosa</name>
</author>
<author><name sortKey="Axthelm, Mk" uniqKey="Axthelm M">MK Axthelm</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Scinicariello, F" uniqKey="Scinicariello F">F Scinicariello</name>
</author>
<author><name sortKey="Engleman, Cn" uniqKey="Engleman C">CN Engleman</name>
</author>
<author><name sortKey="Jayashankar, L" uniqKey="Jayashankar L">L Jayashankar</name>
</author>
<author><name sortKey="Mcclure, Hm" uniqKey="Mcclure H">HM Mcclure</name>
</author>
<author><name sortKey="Attanasio, R" uniqKey="Attanasio R">R Attanasio</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Pelat, T" uniqKey="Pelat T">T Pelat</name>
</author>
<author><name sortKey="Bedouelle, H" uniqKey="Bedouelle H">H Bedouelle</name>
</author>
<author><name sortKey="Rees, Ar" uniqKey="Rees A">AR Rees</name>
</author>
<author><name sortKey="Crennell, Sj" uniqKey="Crennell S">SJ Crennell</name>
</author>
<author><name sortKey="Lefranc, Mp" uniqKey="Lefranc M">MP Lefranc</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hwang, Wy" uniqKey="Hwang W">WY Hwang</name>
</author>
<author><name sortKey="Foote, J" uniqKey="Foote J">J Foote</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Leonard, Jp" uniqKey="Leonard J">JP Leonard</name>
</author>
<author><name sortKey="Friedberg, Jw" uniqKey="Friedberg J">JW Friedberg</name>
</author>
<author><name sortKey="Younes, A" uniqKey="Younes A">A Younes</name>
</author>
<author><name sortKey="Fisher, D" uniqKey="Fisher D">D Fisher</name>
</author>
<author><name sortKey="Gordon, Li" uniqKey="Gordon L">LI Gordon</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rosenwasser, Lj" uniqKey="Rosenwasser L">LJ Rosenwasser</name>
</author>
<author><name sortKey="Busse, Ww" uniqKey="Busse W">WW Busse</name>
</author>
<author><name sortKey="Lizambri, Rg" uniqKey="Lizambri R">RG Lizambri</name>
</author>
<author><name sortKey="Olejnik, Ta" uniqKey="Olejnik T">TA Olejnik</name>
</author>
<author><name sortKey="Totoritis, Mc" uniqKey="Totoritis M">MC Totoritis</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Pelat, T" uniqKey="Pelat T">T Pelat</name>
</author>
<author><name sortKey="Thullier, P" uniqKey="Thullier P">P Thullier</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="De Marco, D" uniqKey="De Marco D">D De Marco</name>
</author>
<author><name sortKey="Clementi, N" uniqKey="Clementi N">N Clementi</name>
</author>
<author><name sortKey="Mancini, N" uniqKey="Mancini N">N Mancini</name>
</author>
<author><name sortKey="Solforosi, L" uniqKey="Solforosi L">L Solforosi</name>
</author>
<author><name sortKey="Moreno, Gj" uniqKey="Moreno G">GJ Moreno</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fodor, E" uniqKey="Fodor E">E Fodor</name>
</author>
<author><name sortKey="Devenish, L" uniqKey="Devenish L">L Devenish</name>
</author>
<author><name sortKey="Engelhardt, Og" uniqKey="Engelhardt O">OG Engelhardt</name>
</author>
<author><name sortKey="Palese, P" uniqKey="Palese P">P Palese</name>
</author>
<author><name sortKey="Brownlee, Gg" uniqKey="Brownlee G">GG Brownlee</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bradbury, Arm" uniqKey="Bradbury A">ARM Bradbury</name>
</author>
<author><name sortKey="Marks, Jd" uniqKey="Marks J">JD Marks</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rowe, T" uniqKey="Rowe T">T Rowe</name>
</author>
<author><name sortKey="Abernathy, Ra" uniqKey="Abernathy R">RA Abernathy</name>
</author>
<author><name sortKey="Hu Primmer, J" uniqKey="Hu Primmer J">J Hu-Primmer</name>
</author>
<author><name sortKey="Thompson, Ww" uniqKey="Thompson W">WW Thompson</name>
</author>
<author><name sortKey="Lu, X" uniqKey="Lu X">X Lu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kendal, Ap" uniqKey="Kendal A">AP Kendal</name>
</author>
<author><name sortKey="Skehel, Jj" uniqKey="Skehel J">JJ Skehel</name>
</author>
<author><name sortKey="Pereira, Ms" uniqKey="Pereira M">MS Pereira</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lambkin, R" uniqKey="Lambkin R">R Lambkin</name>
</author>
<author><name sortKey="Mclain, L" uniqKey="Mclain L">L McLain</name>
</author>
<author><name sortKey="Jones, Se" uniqKey="Jones S">SE Jones</name>
</author>
<author><name sortKey="Aldridge, Sl" uniqKey="Aldridge S">SL Aldridge</name>
</author>
<author><name sortKey="Dimmock, Nj" uniqKey="Dimmock N">NJ Dimmock</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zheng, Bj" uniqKey="Zheng B">BJ Zheng</name>
</author>
<author><name sortKey="Chan, Kw" uniqKey="Chan K">KW Chan</name>
</author>
<author><name sortKey="Lin, Yp" uniqKey="Lin Y">YP Lin</name>
</author>
<author><name sortKey="Zhao, Gy" uniqKey="Zhao G">GY Zhao</name>
</author>
<author><name sortKey="Chan, C" uniqKey="Chan C">C Chan</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<affiliations><list><country><li>République populaire de Chine</li>
</country>
<region><li>Guangdong</li>
</region>
<settlement><li>Jiangmen</li>
</settlement>
</list>
<tree><country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Meng, Weixu" sort="Meng, Weixu" uniqKey="Meng W" first="Weixu" last="Meng">Weixu Meng</name>
</region>
<name sortKey="Chen, Ling" sort="Chen, Ling" uniqKey="Chen L" first="Ling" last="Chen">Ling Chen</name>
<name sortKey="Chen, Ling" sort="Chen, Ling" uniqKey="Chen L" first="Ling" last="Chen">Ling Chen</name>
<name sortKey="Dong, Zhenyuan" sort="Dong, Zhenyuan" uniqKey="Dong Z" first="Zhenyuan" last="Dong">Zhenyuan Dong</name>
<name sortKey="Dong, Zhenyuan" sort="Dong, Zhenyuan" uniqKey="Dong Z" first="Zhenyuan" last="Dong">Zhenyuan Dong</name>
<name sortKey="Feng, Liqiang" sort="Feng, Liqiang" uniqKey="Feng L" first="Liqiang" last="Feng">Liqiang Feng</name>
<name sortKey="Feng, Liqiang" sort="Feng, Liqiang" uniqKey="Feng L" first="Liqiang" last="Feng">Liqiang Feng</name>
<name sortKey="Hu, Xiangjing" sort="Hu, Xiangjing" uniqKey="Hu X" first="Xiangjing" last="Hu">Xiangjing Hu</name>
<name sortKey="Li, Chufang" sort="Li, Chufang" uniqKey="Li C" first="Chufang" last="Li">Chufang Li</name>
<name sortKey="Li, Zhengfeng" sort="Li, Zhengfeng" uniqKey="Li Z" first="Zhengfeng" last="Li">Zhengfeng Li</name>
<name sortKey="Li, Zhengfeng" sort="Li, Zhengfeng" uniqKey="Li Z" first="Zhengfeng" last="Li">Zhengfeng Li</name>
<name sortKey="Luo, Qinfang" sort="Luo, Qinfang" uniqKey="Luo Q" first="Qinfang" last="Luo">Qinfang Luo</name>
<name sortKey="Meng, Weixu" sort="Meng, Weixu" uniqKey="Meng W" first="Weixu" last="Meng">Weixu Meng</name>
<name sortKey="Pan, Weiqi" sort="Pan, Weiqi" uniqKey="Pan W" first="Weiqi" last="Pan">Weiqi Pan</name>
<name sortKey="Pan, Weiqi" sort="Pan, Weiqi" uniqKey="Pan W" first="Weiqi" last="Pan">Weiqi Pan</name>
<name sortKey="Sun, Caijun" sort="Sun, Caijun" uniqKey="Sun C" first="Caijun" last="Sun">Caijun Sun</name>
<name sortKey="Sun, Caijun" sort="Sun, Caijun" uniqKey="Sun C" first="Caijun" last="Sun">Caijun Sun</name>
<name sortKey="Wei, Guowei" sort="Wei, Guowei" uniqKey="Wei G" first="Guowei" last="Wei">Guowei Wei</name>
<name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name>
<name sortKey="Zhang, Anna J X" sort="Zhang, Anna J X" uniqKey="Zhang A" first="Anna J. X." last="Zhang">Anna J. X. Zhang</name>
<name sortKey="Zhong, Nanshan" sort="Zhong, Nanshan" uniqKey="Zhong N" first="Nanshan" last="Zhong">Nanshan Zhong</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001877 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001877 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    SrasV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     PMC:3688872
   |texte=   Rapid Generation of Human-Like Neutralizing Monoclonal Antibodies in Urgent Preparedness for Influenza Pandemics and Virulent Infectious Diseases
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:23824680" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a SrasV1

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 28 14:49:16 2020. Site generation: Sat Mar 27 22:06:49 2021

Serveur d'exploration SRAS

Rapid Generation of Human-Like Neutralizing Monoclonal Antibodies in Urgent Preparedness for Influenza Pandemics and Virulent Infectious Diseases

Rapid Generation of Human-Like Neutralizing Monoclonal Antibodies in Urgent Preparedness for Influenza Pandemics and Virulent Infectious Diseases

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration SRAS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.